Week of 3/1


Introduction

This week, we ran a gel electrophoresis on our amplified DNA samples of D. aquaticus from the

PCR reaction ran the prior week. The fragments we used were intended to be 7kb long, so the

expected result from the gel electrophoresis was two bands measured at the 7kb mark of the

ladder. This result would indicate that we were able to successfully isolate the 7kb plasmid.

Results

The results from the gel electrophoresis showed areas where the dye was smeared across the

gel, and no visible bands could be found at the 7kb mark. Bands were visible at two other spots,

much lower than 7kb.

Discussion

These results indicate that the DNA sample isolated is much shorter in base pairs than we

intended. The use of gibson assembly, a method allowing for the joining of several DNA

fragments into one, will be used the following week in attempts to achieve 7kb plasmid isolation

without having to restart the process from inoculation onward.

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