Week of 4/15

Introduction


This week we reran our UV tests on D. xinjiangensis using R2B media only to grow the bacteria. The purpose of this is to simplify our prior experiment and reduce the amount of variables. We also ran a practice gel electrophoresis.


Procedure


The procedure for the UV test is as follows:

  • Two R2B flasks were inoculated with D. xinjiangensis 

  • In the following days, bacteria samples were concentrated to an OD600 value of 1

  • For each tube sample, a small amount was aseptically transferred onto pieces of parafilm. For each tube, several different samples were placed on parafilm to be exposed to different concentrations of ultraviolet radiation (30000µJ/cm2 and 100000µJ/cm2)

  • Cell samples were subjected to ultraviolet exposure via the UV Crosslinker

  • Afterwards, the parafilm was aseptically scraped and the potential bacteria inoculated into recovery broth, then incubated

  • After 2 days, the broths were inoculated onto properly labeled R2A plates


Results

Results are to be seen in the following week.

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